Rooting and Seedling Strengthening Techniques of Tissue Culture Seedlings of Pseudosasa japonica f. akebonosuji
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摘要: 花叶矢竹是一种珍稀观赏竹，以花叶矢竹愈伤组织脱分化的无根试管苗为试验材料，研究NAA、IBA、6-BA、TDZ以及激素组合对花叶矢竹不定根诱导和壮苗生长的影响。结果表明：NAA对花叶矢竹不定根诱导有主导作用，效果优于单独添加IBA、远优于组合添加NAA、IBA、6-BA。NAA浓度0.5~2.0 mg·L-1时50 d生根率均可达100%，其中浓度0.5 mg·L-1芽苗生根最快，长势最好，15 d生根率已达80%，30 d生根率达100%，平均生根数6.95条，平均根长0.995 cm。NAA浓度0.2~0.5 mg·L-1时，壮苗效果好，且增殖系数可达8.5，平均新芽长可达1.595 cm。添加活性炭、6-BA、TDZ对生根和竹苗生长有抑制作用。Abstract: The effects of NAA, IBA, 6-BA and TDZ on adventitious root induction and seedling strength of Pseudosasa japonica f. Akebonosuji were studied by using tissue culture seedlings as experimental materials. The results showed that NAA played a leading role in adventitious root induction, and the effect was better than that of IBA alone, much better than that of the combination of NAA, IBA and 6-BA. When the concentration of NAA was 0.5-2.0 mg·L-1, the rooting rate reached 100% in 50 days. Under the concentration of 0.5 mg·L-1, the rooting rate was 80% in 15 days and 100% in 30 days. The average rooting number was 6.95 and the average root length was 0.995cm. When NAA concentration was 0.2-0.5 mg·L-1, the seedling effect was good, the multiplication coefficient was up to 8.5, and the average new bud length amounted to 1.595cm.The addition of activated carbon, 6-BA or TDZ could inhibit rooting and proliferation.
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