Abstract: 【Objective】In order to comprehend the shear performance of bamboo scrimber and apply it to structural design safely and efficiently. 【Method】Shear tests on three groups of small specimens of bamboo scrimber under different working conditions were tested in this study, and the mechanical properties and stress—strain relationship of perpendicular to grain, parallel to grain and in plane shears of reconstituted bamboo scrimber were studied. 【Result】The results showed that bamboo scrimber had the highest shear perpendicular to grain strength, with an average value of 15.49 MPa, which was around 2.1 times that of shear parallel to grain strength and 2.7 times that of shear in plane strength. The specimens were classified according to the failure mode, and the relevant stress—strain expression was proposed. The calculation curve was well matched with the test curve. The test results were statistically analyzed using both parametric and non-parametric methods. 【Conclusion】Based on the test values, the 5% quartile value and the parameter method tolerance limit (PTL) under the 75% confidence level were calculated, and the PTL is considered to better reflect the shearing mechanical characteristics of bamboo scrimber.